Carbon/Nitrogen detection experiments for sequential assignments

• CAN correlation experiments
• Ca-detected CAN
K. Takeuchi, Z.-Y. J. Sun, G. Wagner: Alternate 13C-12C labeling for complete mainchain resonance assignments using C alpha direct-detection with applicability toward fast relaxing protein systems. J. Am. Chem. Soc., 130, 17210-17211 (2008), PMC2626166
• c_can_2h_2d.kt
  Ca-detected CAN 2D for alternate 13C-12C proteinlinear-sampling
• c_can_2h_2d_nl.kt
  Ca-detected CAN 2D for alternate 13C-12C proteinnonlinear-sampling
• 3DCANCA with two experiments for uniform and alternate labelingli>
  Takeuchi, K, Frueh, DP, Hyberts, SG, Sun, Z-Y, Wagner, G: High-resolution 3D CANCA NMR experiments for complete mainchain assignments using Ca direct-detection. J Am Chem Soc., 132, 2945-2951 (2010). PMC2832079
  • c_can_2h_3dctsct.ktdf
    Ca-detected CANCA 3D for alternate 13C-12C protein withlinear/nonlinear-sampling and CT/SCT switch
  • c_can_ia_ctsct.ktdf
    Ca-detected CANCA 3D for ul-DCN protein withlinear/nonlinear-sampling and CT/SCT switch
• 15N detected CAN
  K. Takeuchi, G. Heffron, Z.-Yu J. Sun, D.P. Frueh, G. Wagner: Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments. J. Biomol. NMR, 47, 271-282 (2010). PMC2946331
  • n_can_2h_2d.kt
    15N detected CAN for alternate 13C-12C protein with CT/SCT switch
  • n_can_2h_ct_2d_ul.kt
    15N detected CAN for ul-DCN protein
• Fast hCaN
  M. Gal, K. A. Edmonds, A. G. Milbradt, K. Takeuchi, G. Wagner: Speeding up 15N detection: hCaN 2D NMR experiment. J. Biomol. NMR 2011 Dec;51(4):497-504
  • hCaN
• CA TOCSY experiments
  • CACA-TOCSY
    K. Takeuchi, D. P. Frueh, Z.-Yu J. Sun, S. Hiller, G. Wagner: CACA-TOCSY with alternate 13C-12C labeling: a 13C± direct detection experiment for mainchain resonance assignment, dihedral angle information, and amino acid type identification. J. Biomol. NMR, 47, 55-63 (2010). PMC2868269
  • 1H-detected HNCA-TOCSY-CANH
    K. Takeuchi, M. Gal, H. Takahashi, I. Shimada, G. Wagner: HNCA-TOCSY-CANH experiments with alternate 13C-12C labeling: a set of 3D experiment with unique supra-sequential information for mainchain resonance assignment. J. Biomol. NMR, 49, 17-26 (2011). PMC3072286
    • h_N_catoccaNH3d.kt (N evolution)
    • H_ncatoccaNH3d-nonCT.kt (H evolution)
    • hn_CA_toccaNH3d.kt (CA evolution)