Pulse Programs at HMS - README
Welcome to the web portal for our published pulse sequences. In the tabs above you will find pulse sequences not commonly found elsewhere. If you have come here after reading one of our papers and are hoping to find the sequence and/or guidance in getting it up and running, you have come to the right place. Our legacy site is still on line, and can be found here
Most sequences come with a minimal set of instructions in getting things up and running. If you are having trouble feel free to ask for assistance (email Scott Robson... email address found on our main web page) and we'll see what we can do to help.
Below is a short list of what you can find in the tabs above:
HNNH correlation experiments: (updated May 2012)
These experiments are typically of the type hN(coca)NH, Hn(coca)NH or hNCAnH for CA,N,H backbone walk stairway assignment (JACS 2009)
Time-shared experiments: (updated May 2012)
Time-shared NOESY experiments (Frueh et al., J Biomol NMR, 2006, 34, 31-40), Time-shared HSQC-NOESY (NUS) experiments (Frueh et al., J Biomol NMR, 2009) and Time-shared HNCAHA/HACANH correlation experiment (Frueh et al., J Biomol NMR, 2005, 33, 187-96)
15N/13C detected experiments (updated May 2012)
CAN correlation experiments and CA TOCSY experiments
Miscellaneous experiments: (coming soon)
Maybe what you are looking for is here.
Publications: (coming soon)
Links to publications can be found here.
We have and continue to develop Non uniform sampling procedures with all our sequences. Many of the above sequences have been made Non-uniform compatible. However, because of the nature of acquisition of non uniform sampling spectra (especially on Bruker instruments) there is often no one method that works on all spectrometers. Also, the acquistion method is tightly coupled to how data is ultimately reconstructed and transformed. Because of this we have devoted an entirely new web portal to distribute our non-uniform sequences and descriptions of how to get them working and how to reconstruct and transform them. This portal features many non-uniform sampling pulse sequences and a description of how to leverage our new methodology (iterative soft threshold, IST) for reconstruction.
Missing information?
We aim to keep this information up to date and as useful as possible. If you think anything needs to be updated, again contact Scott Robson. This includes published sequences that are missing here or instructions that don't make sense. Keep in mind we expect you have some level of comfort with running non-standard sequecnes on Varian and Bruker instruments - we can't help with basic spectrometer problems.